1O7F
CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.429, 96.062, 103.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27800 |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.700 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 * | 0.170 * |
| Total number of observations | 151369 * | |
| Number of reflections | 23875 | |
| <I/σ(I)> | 24.4 | 7.8 |
| Completeness [%] | 96.8 | 92.9 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 25 MM HEPES PH 7.5, 1M NA K PHOSPHATE, 10 MM DTE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 25 (mM) | pH7.5 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | 750 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | protein | 40 (mg/ml) |
