1O70
Novel Fold Revealed by the Structure of a FAS1 Domain Pair from the Insect Cell Adhesion Molecule Fasciclin I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-15 |
Detector | ADSC CCD |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 150.806, 150.806, 150.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26300 * |
Structure solution method | MIR |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.200 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.058 | 0.309 * |
Number of reflections | 17949 | |
<I/σ(I)> | 4.4 | 2.4 |
Completeness [%] | 97.1 | 97.5 |
Redundancy | 8.4 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 0.1 M TRIS-HCL PH 8.5, 2.0 M AMMONIUM SULFATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | sodium HEPES | 0.01 (M) | pH7.5 |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
4 | 1 | reservoir | ammonium sulfate | 2.0 (M) |