1O6S
Internalin (Listeria monocytogenes) / E-Cadherin (human) Recognition Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.378, 86.859, 110.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.000 * - 1.800 |
R-factor | 0.171 |
Rwork | 0.168 |
R-free | 0.22200 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 * |
RMSD bond angle | 1.780 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.385 |
Number of reflections | 46306 * | |
<I/σ(I)> | 14.4 | 3.4 |
Completeness [%] | 92.0 | 84.3 |
Redundancy | 6.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | VAPOUR DIFFUSION / HANGING DROP 10 MG/ML IN 10 MM HEPES PH 7.0, 26% PEG 4000, 100 MM MES/TRIS PH 7.0, 100 MM SODIUM ACETATE, 50 MM CACL2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 26 (%) | |
3 | 1 | reservoir | MES-Tris | 0.1 (M) | pH7.0 |
4 | 1 | reservoir | sodium acetate | 100 (mM) | |
5 | 1 | reservoir | 50 (mM) |