1O69
Crystal structure of a PLP-dependent enzyme
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 151.774, 151.774, 76.986 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.280 - 1.840 |
| Rwork | 0.185 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA) |
| Refinement software | REFMAC (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.220 | 1.950 |
| High resolution limit [Å] | 1.840 * | 1.850 |
| Rmerge | 0.071 | 0.680 |
| Number of reflections | 84957 | |
| <I/σ(I)> | 20.5 | 4 |
| Completeness [%] | 98.3 | 89.6 |
| Redundancy | 13.9 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 285 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | protein | 9.6mg/ml | |
| 2 | 1 | 2 | NaCl | 150mM | |
| 3 | 1 | 2 | Hepes | 20mM | |
| 4 | 1 | 2 | Methionine | 10mM | |
| 5 | 1 | 2 | beta-mercaptoethanol | 1mM |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | methionine | 10 (mM) | |
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
