1O5J
Crystal structure of Periplasmic divalent cation tolerance protein (TM1056) from Thermotoga maritima at 1.95 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-25 |
| Detector | APS |
| Wavelength(s) | 0.97944, 0.97924, 0.95658 |
| Spacegroup name | P 3 |
| Unit cell lengths | 52.514, 52.514, 34.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.480 - 1.950 |
| R-factor | 0.15907 |
| Rwork | 0.156 |
| R-free | 0.22061 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.441 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.480 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 7337 | |
| <I/σ(I)> | 32.91 | 3.7 |
| Completeness [%] | 100.0 | 70.02 |
| Redundancy | 7.49 | 3.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 10.5 | 293 | 30% PEG-400, 0.1M CAPS pH 10.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
