1O5I
Crystal structure of 3-oxoacyl-(acyl carrier protein) reductase (TM1169) from Thermotoga maritima at 2.50 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.515, 117.120, 140.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.170 - 2.500 |
| R-factor | 0.19662 |
| Rwork | 0.194 |
| R-free | 0.24680 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i01 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.755 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.170 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 31633 | |
| <I/σ(I)> | 7.7 | 1.6 |
| Completeness [%] | 85.4 | 42.7 |
| Redundancy | 2.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5 | 293 | 20% PEG-6000, 0.1M citric acid pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
