1O5H
Crystal structure of formiminotetrahydrofolate cyclodeaminase (TM1560) from Thermotoga maritima at 2.80 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-21 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.704, 71.875, 119.245 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.000 - 2.800 |
R-factor | 0.20289 |
Rwork | 0.199 |
R-free | 0.27800 * |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.460 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA (5.0)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.670 |
High resolution limit [Å] | 2.800 * | 2.800 * |
Rmerge | 0.067 * | 0.470 * |
Total number of observations | 81783 * | |
Number of reflections | 12284 * | |
<I/σ(I)> | 13.3 | 1.4 |
Completeness [%] | 97.1 * | 76.5 * |
Redundancy | 6.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.9 * | 277 | 5% PEG-6000, 0.1M citric acid pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 20 (mM) | pH7.9 |
2 | 1 | reservoir | 150 (mM) | ||
3 | 1 | reservoir | TCEP | 0.25 (mM) | |
4 | 1 | drop | protein | 12 (mg/ml) |