1O59
Crystal structure of Allantoicase (yir029w) from Saccharomyces cerevisiae at 2.40 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-17 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 107.209, 107.209, 134.918 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.970 - 2.400 |
R-factor | 0.17765 |
Rwork | 0.175 |
R-free | 0.22200 * |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.510 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.970 * | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.099 | 0.707 |
Total number of observations | 94513 * | |
Number of reflections | 18532 | |
<I/σ(I)> | 13 | 1.8 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 5.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.8 * | 293 | 30% Ethylene Glycol , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
1 | Vapor diffusion * | 7.8 * | 293 | 30% Ethylene Glycol , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
1 | Vapor diffusion * | 7.8 * | 293 | 30% Ethylene Glycol , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 10 (mM) | pH7.8 |
2 | 1 | reservoir | 150 (mM) | ||
3 | 1 | drop | protein | 8 (mg/ml) |