1O54
Crystal structure of SAM-dependent O-methyltransferase (TM0748) from Thermotoga maritima at 1.65 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 64.253, 74.992, 143.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.930 - 1.650 |
R-factor | 0.15918 |
Rwork | 0.158 |
R-free | 0.18762 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i9g |
RMSD bond length | 0.015 |
RMSD bond angle | 1.409 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.930 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 40334 | |
<I/σ(I)> | 17.4 | 1.9 |
Completeness [%] | 95.9 | 78.8 |
Redundancy | 4.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5 | 293 | 40% MPD, 0.1M sodium acetate trihydrate, pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |