1O51
Crystal structure of a putative PII-like signaling protein (TM0021) from Thermotoga maritima at 2.50 A resolution
Replaces: 1O2CExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-27 |
Detector | APS-1 |
Wavelength(s) | 0.97914, 0.97934 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 100.110, 100.110, 100.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.710 * - 2.500 |
R-factor | 0.19 |
Rwork | 0.184 |
R-free | 0.22900 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.780 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.710 * | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.085 * | 0.499 * |
Total number of observations | 126573 * | |
Number of reflections | 6360 * | |
<I/σ(I)> | 70.05 | 10.64 |
Completeness [%] | 100.0 | 100 |
Redundancy | 43.83 | 0.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.9 * | 293 | 2M ammonium sulfate, 0.1M tri-sodium citrate dihydrate pH 5.6, 0.2M potassium sodium tartrate tetrahydrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 5.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 20 (mM) | pH7.9 |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | TCEP | 0.25 (mM) | |
4 | 1 | drop | protein | 17 (mg/ml) | |
5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
6 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
7 | 1 | reservoir | citrate | 0.1 (M) | pH5.6 |