1O1Y
Crystal structure of a glutamine amidotransferase (tm1158) from thermotoga maritima at 1.70 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-26 |
Detector | APS-1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.362, 92.362, 135.833 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 79.100 * - 1.700 |
R-factor | 0.14651 |
Rwork | 0.145 |
R-free | 0.17200 * |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.520 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.100 * | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.087 * | 0.643 * |
Total number of observations | 314408 * | |
Number of reflections | 36314 | |
<I/σ(I)> | 16 | 1.7 |
Completeness [%] | 94.9 | 72.8 |
Redundancy | 8.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.9 * | 293 | 2.4 M Ammonium Sulfate; 0.1 M Bicine pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
1 | Vapor diffusion * | 7.9 * | 293 | 2.4 M Ammonium Sulfate; 0.1 M Bicine pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
1 | Vapor diffusion * | 7.9 * | 293 | 2.4 M Ammonium Sulfate; 0.1 M Bicine pH 9.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
2 | 1 | reservoir | Bicine | 0.1 (M) | pH9.0 |
3 | 1 | drop | Tris | 20 (mM) | pH7.9 |
4 | 1 | drop | 150 (mM) | ||
5 | 1 | drop | TCEP | 0.25 (mM) |