1O1X
Crystal structure of a ribose 5-phosphate isomerase rpib (tm1080) from thermotoga maritima at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91837, 0.97931, 0.97892 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.490, 73.890, 87.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.790 * - 1.900 |
| R-factor | 0.118 |
| Rwork | 0.117 |
| R-free | 0.13800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.520 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.790 * | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 * | 0.284 * |
| Total number of observations | 75731 * | |
| Number of reflections | 15973 | |
| <I/σ(I)> | 15 | 3.4 |
| Completeness [%] | 99.1 | 93.2 |
| Redundancy | 4.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 277 | 40 % (+/-)-2-Methyl-2,4-Pentanediol; 0.1 M HEPES pH 7.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.9 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | TCEP | 0.25 (mM) | |
| 4 | 1 | drop | protein | 12 (mg/ml) | |
| 5 | 1 | reservoir | MPD | 40 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
