1O1K
Deoxy hemoglobin (A,C:V1M; B,D:V1M,V67W)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.470, 83.627, 53.644 |
| Unit cell angles | 90.00, 99.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| Rwork | 0.216 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c7c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.273 |
| Number of reflections | 26599 | |
| <I/σ(I)> | 12.6 | 3.68 |
| Completeness [%] | 71.0 | 75.9 |
| Redundancy | 2.17 | 1.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.50 |
