Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998 |
Detector | MARRESEARCH |
Wavelength(s) | 0.834 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 92.340, 65.850, 116.440 |
Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
Resolution | 18.000 - 2.050 |
Rwork | 0.213 |
R-free | 0.26000 |
Structure solution method | MAD |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.053 * | 0.242 * |
Number of reflections | 77715 | 3382 * |
<I/σ(I)> | 10.8 | 1.8 |
Completeness [%] | 92.7 | 81.2 |
Redundancy | 3.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | Ivens, A., (2001) Eur.J.Biochem., 268, 2246. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6-8 (mg/ml) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
3 | 1 | reservoir | PEG1500 | 12-18 (%) |