1O0X
Crystal structure of Methionine aminopeptidase (TM1478) from Thermotoga maritima at 1.90 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-29 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.580, 62.580, 146.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT WITH PROGRAM AMORE |
| Starting model (for MR) | 1mat |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.470 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 * | 0.614 * |
| Total number of observations | 102128 * | |
| Number of reflections | 21470 | |
| <I/σ(I)> | 16.53 | 2.95 |
| Completeness [%] | 90.6 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.9 * | 293 | 19% iso-propanol, 19% P4K, 0.095 M Na-citrate pH 5.6, 5% glycerol, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.9 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | TCEP | 0.25 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | isopropanol | 19 (%) | |
| 6 | 1 | reservoir | PEG4000 | 19 (%) | |
| 7 | 1 | reservoir | sodium citrate | 0.095 (M) | pH5.6 |
| 8 | 1 | reservoir | glycerol | 5 (%) |
