1O0W
Crystal structure of Ribonuclease III (TM1102) from Thermotoga maritima at 2.0 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979321, 0.978964, 0.918370 |
Spacegroup name | P 1 |
Unit cell lengths | 36.000, 58.500, 70.200 |
Unit cell angles | 67.20, 92.00, 77.70 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.23000 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.380 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.899 | 2.100 |
High resolution limit [Å] | 1.995 | 1.990 |
Number of reflections | 32670 | |
<I/σ(I)> | 11.6 | 3.8 |
Completeness [%] | 93.7 | 88.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.9 | 293 | 0.2 M Potassium Nitrate, 20% PEG 3350, pH 6.9, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 6.90 |