1O0T

CRYSTAL STRUCTURE OF A COLD ADAPTED ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18, CO-CRYSTALLIZED WITH 5 mM EDTA (5 DAYS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	2002-02-05
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	185.700, 185.700, 38.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.184
Rwork	0.167
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g9k
RMSD bond length	0.010
RMSD bond angle	1.490
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.116 *	0.374 *
Total number of observations	33198 *
Number of reflections	16749
Completeness [%]	99.1	98.3
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	Villeret, V., (1997) Protein Sci., 6, 2462. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.6 (M)
2	1	reservoir	HEPES	0.1 (M)	pH7.0
3	1	drop	protein	15 (mg/ml)