1O0O
Ribonuclease A in complex with adenosine-2',5'-diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00061 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.852, 32.528, 72.383 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 29.360 - 1.200 |
| R-factor | 0.19431 |
| Rwork | 0.194 |
| R-free | 0.22800 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.038 * | 0.226 * |
| Total number of observations | 949552 * | |
| Number of reflections | 67242 | |
| <I/σ(I)> | 30.9 | |
| Completeness [%] | 92.9 * | 71.2 |
| Redundancy | 14.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 16 * | Leonidas, D.D., (1997) Biochemistry, 36, 5578. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 10 (mM) | |
| 3 | 1 | drop | PEG4000 | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | sodium citrate | 20 (M) | pH5.5 |
