1O0H
Ribonuclease A in complex with 5'-ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.722, 32.515, 72.164 |
Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
Resolution | 29.240 - 1.200 |
R-factor | 0.18963 |
Rwork | 0.190 |
R-free | 0.21900 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1afu |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.053 * | 0.130 * |
Total number of observations | 623242 * | |
Number of reflections | 70118 | |
<I/σ(I)> | 20.9 | |
Completeness [%] | 96.5 | 96.9 |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 16 * | Leonidas, D.D., (1997) Biochemistry, 36, 5578. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium citrate | 10 (mM) | |
3 | 1 | drop | PEG4000 | 10 (%) | |
4 | 1 | reservoir | PEG4000 | 20 (%) | |
5 | 1 | reservoir | sodium citrate | 20 (M) | pH5.5 |