1O0F
RNASE A in complex with 3',5'-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5419 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.862, 32.694, 71.930 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 29.020 - 1.500 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 23.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 * | 0.369 * |
| Total number of observations | 423949 * | |
| Number of reflections | 36948 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 97.9 | 95.5 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 16 * | Leonidas, D.D., (1997) Biochemistry, 36, 5578. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 10 (mM) | |
| 3 | 1 | drop | PEG4000 | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | sodium citrate | 20 (M) | pH5.5 |
