1O0E
1.9 Angstrom Crystal Structure of a plant cysteine protease Ervatamin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.729, 82.691, 133.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.900 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.560 |
| Total number of observations | 157915 * | |
| Number of reflections | 38978 | |
| <I/σ(I)> | 20.15 | 2.86 |
| Completeness [%] | 97.9 | 99.8 |
| Redundancy | 4.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG8000, potassium phosphate monobasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 0.01 (M) | pH7.0 |
| 3 | 1 | reservoir | potassium dihydrogen orhtophosphate | 0.05 (M) | |
| 4 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |
