1O0A
BACTERIORHODOPSIN L INTERMEDIATE AT 1.62 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-27 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.801, 60.801, 107.778 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.620 |
| Rwork | 0.154 |
| R-free | 0.19900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m0l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.690 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.058 | 0.593 |
| Total number of observations | 572564 * | |
| Number of reflections | 28097 | |
| <I/σ(I)> | 36.1 | 2.4 |
| Completeness [%] | 99.7 | 88.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 5.6 | 20 * | Gabriele, R., (1998) J. Struct. Biol., 121, 82. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3.3 (mg/ml) | |
| 2 | 1 | 1 | sodium potassium Pi | 1 (M) | |
| 3 | 1 | 1 | MPD | 2.5 (%) |
