1NZO
The crystal structure of wild type penicillin-binding protein 5 from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-01-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.350, 50.280, 84.530 |
Unit cell angles | 90.00, 120.90, 90.00 |
Refinement procedure
Resolution | 14.920 - 1.850 |
R-factor | 0.20964 |
Rwork | 0.208 |
R-free | 0.24503 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hd8 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.509 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.323 |
Number of reflections | 31624 | |
<I/σ(I)> | 7.4 | 2 |
Completeness [%] | 93.4 | 67.8 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 100mM Tris pH 7.0, 8% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 294K |