1NZJ
Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-08 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.920, 39.300, 69.150 |
| Unit cell angles | 90.00, 116.19, 90.00 |
Refinement procedure
| Resolution | 24.254 - 1.500 |
| R-factor | 0.14729 |
| Rwork | 0.146 |
| R-free | 0.16969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gln |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.020 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.057 | 0.283 |
| Number of reflections | 43648 | |
| <I/σ(I)> | 9.1 | 2.5 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG4000, MPD, Nacacodylate, pH 6.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
