1NZG
Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.260, 44.110, 45.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.600 |
| Rwork | 0.192 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.063 | 0.182 |
| Number of reflections | 7031 | |
| <I/σ(I)> | 22.9 | 6.5 |
| Completeness [%] | 97.6 | 82.9 |
| Redundancy | 8.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | MPD, Sodium Cacodylate, Spermine, Sodium Chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Sodium Cacodylate | ||
| 3 | 1 | 1 | Spermine | ||
| 4 | 1 | 1 | Sodium Chloride | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | Sodium Chloride |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2000 (nm) | |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
| 3 | 1 | drop | 80 (mM) | ||
| 4 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
| 5 | 1 | drop | 10 (%(v/v)) | ||
| 6 | 1 | reservoir | MPD | 35 (%(v/v)) |
