1NZ6
Crystal Structure of Auxilin J-Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9788, 0.9790, 0.9813 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.066, 103.138, 75.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 20.100 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.049 | |
| Number of reflections | 12866 | |
| <I/σ(I)> | 38.9 | |
| Completeness [%] | 100.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 4 * | PEG 8000, Tris-Cl, TCEP, Glycerol, pH 8.0, microbatch under oil, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15-20 (mg/ml) | |
| 2 | 1 | 1 | Tris | 10 (mM) | pH8.0 |
| 3 | 1 | 1 | TCEP | 3 (mM) | |
| 4 | 1 | 1 | glycerol | 15 (%) | |
| 5 | 1 | 1 | PEG8000 | 12.5 (%) |
