1NZ6
Crystal Structure of Auxilin J-Domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.9788, 0.9790, 0.9813 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 94.066, 103.138, 75.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 * - 2.500 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 20.100 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.049 | |
Number of reflections | 12866 | |
<I/σ(I)> | 38.9 | |
Completeness [%] | 100.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 8 | 4 * | PEG 8000, Tris-Cl, TCEP, Glycerol, pH 8.0, microbatch under oil, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 15-20 (mg/ml) | |
2 | 1 | 1 | Tris | 10 (mM) | pH8.0 |
3 | 1 | 1 | TCEP | 3 (mM) | |
4 | 1 | 1 | glycerol | 15 (%) | |
5 | 1 | 1 | PEG8000 | 12.5 (%) |