1NZ5

The Horse heart myoglobin variant K45E/K63E complexed with Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	28.900, 35.600, 125.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.175
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020 *
RMSD bond angle	0.041 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.700
Rmerge	0.083
Number of reflections	11392
Completeness [%]	77.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	25 *	ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ammonium sulfate	60 (%sat)
3	1	drop	Tris-HCl	20 (mM)
4	1	drop	EDTA	1 (mM)	pH8.3
5	1	reservoir	ammonium sulfate	65 (%sat)
6	1	reservoir	Tris-HCl	20 (mM)
7	1	reservoir	EDTA	1 (mM)