1NYL
Unliganded glutaminyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.400, 64.120, 208.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.400* |
R-factor | 0.251 |
Rwork | 0.251 |
R-free | 0.33400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.230 * |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | |
High resolution limit [Å] | 2.400 * | |
Rmerge | 0.059 * | 0.378 * |
Total number of observations | 141412 * | |
Number of reflections | 21185 * | |
Completeness [%] | 93.5 * | 95.8 * |
Redundancy | 3.7 * | 3.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 290 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG1000 | 10 (%) | |
2 | 1 | reservoir | PEG8000 | 10 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | AMPCPP | 5 (mM) | |
5 | 1 | reservoir | PIPES | 0.1 (M) | pH7.5 |