1NYK

Crystal Structure of the Rieske protein from Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.7373, 1.3776, 1.7415
Spacegroup name	C 2 2 21
Unit cell lengths	58.569, 78.267, 136.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 1.310
R-factor	0.1592
Rwork	0.158
R-free	0.18900 *
Structure solution method	MAD
RMSD bond length	0.012 *
RMSD bond angle	2.280 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.370
High resolution limit [Å]	1.300	1.300 *
Rmerge	0.057 *	0.431 *
Total number of observations	319817 *
Number of reflections	76407 *
Completeness [%]	98.8	93.3 *
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	Peg 4000, Tris, magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	Peg 4000, Tris, magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	25 (%)
3	1	reservoir	Tris	100 (mM)	pH8.5
4	1	reservoir		0.1-0.4 (M)
5	1	reservoir			10000-11000nm