1NYH
Crystal Structure of the Coiled-coil Dimerization Motif of Sir4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-06-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97827, 0.978423, 0.95 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.640, 108.640, 74.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.140 - 3.100 |
| R-factor | 0.27254 |
| Rwork | 0.271 |
| R-free | 0.28800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.184 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.066 | 0.303 |
| Total number of observations | 191300 * | |
| Number of reflections | 4796 | |
| <I/σ(I)> | 33.3 | 9.2 |
| Completeness [%] | 96.2 * | 97.2 |
| Redundancy | 11.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 22 * | PEG 3350, MES, MgCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 350 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | PEG3350 | 1 (%) |
