1NYE
Crystal structure of OsmC from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.529, 90.291, 112.677 |
Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.400 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.27700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 38395 | |
<I/σ(I)> | 25.6 | 2.2 |
Completeness [%] | 98.2 | 97.9 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M magnesium formate, 20%PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |