1NXP
MicArec pH4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.395, 92.694, 36.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.470 - 1.820 |
R-factor | 0.18373 |
Rwork | 0.179 |
R-free | 0.23040 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nxt |
RMSD bond length | 0.020 |
RMSD bond angle | 1.911 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.27) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.470 | 1.867 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.072 * | 0.345 * |
Total number of observations | 199669 * | |
Number of reflections | 11155 | |
Completeness [%] | 98.6 | 99.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 20 * | PEG 300, Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 25 (mM) | |
2 | 1 | drop | 300 (mM) | pH7.5 | |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | reservoir | PEG300 | 30 (%(v/v)) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |