1NXH
X-RAY STRUCTURE: NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET TT87
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-19 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97924 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.223, 80.223, 106.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.800 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.31900 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 8628 | |
Completeness [%] | 96.0 | 78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 293 | 0.1M Tris-HCl 9.2, 0.2M Sodium Acetate, 30% PEG 4000, 2% Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |