1NWY
COMPLEX OF THE LARGE RIBOSOMAL SUBUNIT FROM DEINOCOCCUS RADIODURANS WITH AZITHROMYCIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-11-14 |
| Detector | SBC |
| Wavelength(s) | 1.03320 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 170.800, 409.500, 695.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 3.200* |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.30400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1LNR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.110 * | 0.339 * |
| Number of reflections | 330873 | |
| <I/σ(I)> | 9 | 1.9 |
| Completeness [%] | 86.6 | 83.4 |
| Redundancy | 4.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 18 * | Harms, J.M., (2001) Cell (Cambridge,Mass.), 107, 679. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 10 (mM) | ||
| 2 | 1 | reservoir | 60 (mM) | ||
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | HEPES | 10 (mM) | pH7.8 |
