1NWT
Crystal structure of human cartilage gp39 (HC-gp39) in complex with chitopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 43 |
| Unit cell lengths | 128.320, 128.320, 108.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.114 * | 0.626 * |
| Total number of observations | 227637 * | |
| Number of reflections | 59975 | |
| <I/σ(I)> | 10.1 | 2 |
| Completeness [%] | 97.9 | 96.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | pH 5.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | BES | 10 (mM) | pH7.2 |
| 4 | 1 | reservoir | PEG8000 | 10 (%) | |
| 5 | 1 | reservoir | 0.5 (M) | ||
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.1 |
