1NWQ
CRYSTAL STRUCTURE OF C/EBPALPHA-DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.892, 53.089, 67.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.800 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dh3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 20.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.076 | 0.415 |
| Total number of observations | 222356 * | |
| Number of reflections | 12416 | |
| <I/σ(I)> | 19.2 | 2.7 |
| Completeness [%] | 95.6 | 91.6 |
| Redundancy | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 4 * | NACL, MGCL2, PEG 400, MES, GLYCEROL, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.75 (mM) | |
| 2 | 1 | drop | DNA | 0.5 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | MES | 100 (mM) | pH5.7 |
| 5 | 1 | reservoir | MES | 100 (mM) | pH5.7 |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | 30 (mM) | ||
| 8 | 1 | reservoir | PEG400 | 18 (%) | |
| 9 | 1 | reservoir | glycerol | 10 (%) |
