1NWO
CRYSTALLOGRAPHIC STUDY OF AZURIN FROM PSEUDOMONAS PUTIDA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1990-09 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.250, 50.650, 54.600 |
| Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.920 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AZURIN FROM ALCALIGENES DENITRIFICANS (PDB ENTRY 2AZA) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.200 * |
| Data reduction software | SAME (AS ABOVE) |
| Data scaling software | HAMLIN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.048 | 0.193 |
| Total number of observations | 56890 * | |
| Number of reflections | 16127 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 92.5 | 60 |
| Redundancy | 3.5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HANGING DROP METHOD AT 4 C BY MIXING 5 MICRLITER PROTEIN AT 10- 15MG PER ML WITH 5 MICROLITER 30-36% PEG8000 SOLUTION CONTAINING 5MM TRIS-HCL BUFFER, PH 6.5-7.5 AND 100MM NACL., vapor diffusion - hanging drop, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7.5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 15-18 (%) | |
| 3 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | reservoir | PEG8000 | 30-36 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 5 (mM) | |
| 7 | 1 | reservoir | 100 (mM) |
