1NVY
Strontium bound to the Holliday junction sequence d(TCGGTACCGA)4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9470, 0.7301, 0.7667, 0.7670 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.734, 25.320, 36.493 |
| Unit cell angles | 90.00, 110.11, 90.00 |
Refinement procedure
| Resolution | 34.300 - 1.500 |
| R-factor | 0.20962 |
| Rwork | 0.207 |
| R-free | 0.26294 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.442 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.075 | 0.228 |
| Number of reflections | 16734 | |
| <I/σ(I)> | 13.58 | 4.38 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 1.9 | 1.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | SrCl2, MPD, NaCacodylate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
