1NVN
Structural Characterisation of the Holliday junction formed by the sequence CCGGTACCGG at 1.8 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Wavelength(s) | 0.811 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 66.380, 23.790, 37.120 |
Unit cell angles | 90.00, 110.13, 90.00 |
Refinement procedure
Resolution | 34.920 - 1.800 |
R-factor | 0.20872 |
Rwork | 0.206 |
R-free | 0.26361 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.731 |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.920 | 2.100 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.200 |
Number of reflections | 5158 | |
<I/σ(I)> | 16.78 | 6.06 |
Completeness [%] | 98.3 | 98.4 |
Redundancy | 3.71 | 3.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MPD, CaCl2, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |