1NV8
N5-glutamine methyltransferase, HemK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.489, 59.062, 85.605 |
| Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.200 |
| R-factor | 0.18958 |
| Rwork | 0.186 |
| R-free | 0.25400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 1.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.351 |
| Total number of observations | 176619 * | |
| Number of reflections | 32346 * | |
| <I/σ(I)> | 26 | 2 |
| Completeness [%] | 94.7 | 66.5 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 19 * | PEG 3000, NaCitrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | PEG3000 | 16-20 (%) | |
| 3 | 1 | reservoir | sodium citrate trihydrate | 100 (mM) | pH5.5 |
