1NV2
Fructose-1,6-Bisphosphatase Complex with Magnesium, Fructose-6-Phosphate, Phosphate and Thallium (20 mM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-09 |
Detector | BRANDEIS |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 52.361, 82.765, 165.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.100 |
R-factor | 0.178 |
Rwork | 0.170 |
R-free | 0.24870 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eyi |
RMSD bond length | 0.006 |
RMSD bond angle | 24.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.030 | |
Total number of observations | 92869 * | |
Number of reflections | 17604 | |
Completeness [%] | 86.5 | 43.7 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG 3350, HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | KPi | 10 (mM) | pH7.4 |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | F6P | 5 (mM) | |
5 | 1 | drop | thalium acetate | 20 (mM) | |
6 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
7 | 1 | reservoir | t-butyl alcohol | 5 (%) | |
8 | 1 | reservoir | glycerol | 27 (%(v/v)) | |
9 | 1 | reservoir | PEG3350 | 8 (%(w/v)) |