1NUX
Fructose-1,6-Bisphosphatase Complex with Magnesium, Fructose-6-Phosphate, Phosphate and inhibitory concentrations of Potassium (200mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.813, 82.995, 165.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.158 |
| Rwork | 0.152 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.017 | |
| Total number of observations | 187681 * | |
| Number of reflections | 39885 | |
| Completeness [%] | 83.5 | 35.6 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 295 | PEG 3350, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | F16P2 | 5 (mM) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7. |
| 7 | 1 | reservoir | t-butylalcohol | 5 (%) | |
| 8 | 1 | reservoir | glycerol | 27 (%(v/v)) | |
| 9 | 1 | reservoir | PEG3350 | 8 (%(w/v)) |
