1NUK
CRYSTAL STRUCTURE OF THE LIGAND-BINDING DOMAIN OF THE EPHB2 RECEPTOR TYROSINE KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.150, 55.150, 158.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.31400 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.970 |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.110 |
| Number of reflections | 4214 |
| Completeness [%] | 95.0 |
| Redundancy | 5.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | HEPES | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 15 (%) | |
| 7 | 1 | reservoir | sodium acetate | 50 (mM) |
