1NU5
Crystal structure of Pseudomonas sp. P51 Chloromuconate lactonizing enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99015 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 135.355, 135.355, 105.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.950 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.256 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.171 * |
| Total number of observations | 1063968 * | |
| Number of reflections | 35772 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 * | 277 | PEG 400, Mangane chloride, hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | PEG400 | 12 (%(w/v)) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | Tris | 20 (mM) | pH7.2 |
| 6 | 1 | drop | 2 (mM) | ||
| 7 | 1 | drop | cis,cis-muconate | 1 (mM) |
