1NTY
Crystal structure of the first DH/PH domain of Trio to 1.7 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 99.540, 99.540, 98.294 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.820 - 1.700 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.21400 |
Structure solution method | Molecular Replacement followed by ARP/wARP |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 56048 | |
<I/σ(I)> | 18.8 | 2 |
Completeness [%] | 90.4 | 55.8 |
Redundancy | 24.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch Method. | 7.5 | 277 | DTT, pH 7.5, Batch Method., temperature 277K |