1NTM

Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	154.325, 154.325, 593.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.400
R-factor	0.23808
Rwork	0.237
R-free	0.28200 *
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.015
RMSD bond angle	1.929 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.461
High resolution limit [Å]	2.400	2.400
Rmerge	0.058 *	0.392 *
Number of reflections	119745
Completeness [%]	85.7	77.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	277	PEG 4000, ammonium acetate, potassium chloride, glyerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MOPS	50 (mM)	pH7.2
3	1	reservoir	ammonium acetate	20 (mM)
4	1	reservoir	glycerol	20 (%(w/v))
5	1	reservoir	decanoyl-N-methylglucamide	0.1 (%)