1NTM
Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 154.325, 154.325, 593.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.400 |
| R-factor | 0.23808 |
| Rwork | 0.237 |
| R-free | 0.28200 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.929 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.461 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.058 * | 0.392 * |
| Number of reflections | 119745 | |
| Completeness [%] | 85.7 | 77.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 277 | PEG 4000, ammonium acetate, potassium chloride, glyerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | MOPS | 50 (mM) | pH7.2 |
| 3 | 1 | reservoir | ammonium acetate | 20 (mM) | |
| 4 | 1 | reservoir | glycerol | 20 (%(w/v)) | |
| 5 | 1 | reservoir | decanoyl-N-methylglucamide | 0.1 (%) |
