1NTK
Crystal Structure of Mitochondrial Cytochrome bc1 in Complex with Antimycin A1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 153.785, 153.785, 592.498 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.600 |
R-factor | 0.23421 |
Rwork | 0.233 |
R-free | 0.27200 * |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.019 * |
RMSD bond angle | 1.851 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.667 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.075 * | 0.524 * |
Number of reflections | 107555 | |
Completeness [%] | 98.5 | 95.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 | 277 | PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | MOPS | 50 (mM) | pH7.2 |
3 | 1 | reservoir | ammonium acetate | 20 (mM) | |
4 | 1 | reservoir | glycerol | 20 (%(w/v)) | |
5 | 1 | reservoir | decanoyl-N-methylglucamide | 0.1 (%) |