1NTH
Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)
Replaces: 1L2RExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 158.464, 158.464, 135.797 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| Rwork | 0.176 |
| R-free | 0.18800 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.071 | 0.510 |
| Number of reflections | 140499 | |
| <I/σ(I)> | 16.8 | 2.5 |
| Completeness [%] | 97.3 | 93.7 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 4.3M NACL, 0.1M HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
