1NTG

Crystal Structure of the EMAP II-like Cytokine Released from human tyrosyl-tRNA Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2001-10-30
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	54.621, 59.515, 71.591
Unit cell angles	103.51, 109.95, 101.52

Refinement procedure

Resolution	20.000 - 2.200 *
Rwork	0.251
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EMAP II structure (PDB 1FL0)
RMSD bond length	0.006
RMSD bond angle	1.540
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.290
High resolution limit [Å]	2.200 *	2.210
Rmerge	0.067	0.252
Number of reflections	39281
<I/σ(I)>		3.8
Completeness [%]	94.6	83.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	4 *	ammonium sulfate, Sodium phosphate mono-basic, potassium phosphate di-basic, acetone, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 279K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.8 (M)
2	1	reservoir	sodium potassium phosphate	0.1 (M)	pH6.9
3	1	reservoir	acetone	2 (%)