1NT8
Structural Characterisation of the Holliday junction formed by the sequence CCGGTACCGG at 2.00 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.811 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.949, 23.756, 37.204 |
Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
Resolution | 34.920 - 2.000 |
R-factor | 0.24834 |
Rwork | 0.247 |
R-free | 0.28262 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 3.768 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.920 | 2.010 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.103 | 0.849 |
Number of reflections | 4276 | |
<I/σ(I)> | 4.18 | 0.8 |
Completeness [%] | 97.6 | 97.4 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | MPD, Calcium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |